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KMID : 1007020100080030271
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Korean Soceity of Osteroporosis
2010 Volume.8 No. 3 p.271 ~ p.279
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Roles of Orphan Nuclear Receptor Small Heterodimer Partner in Bone Development: Microcomputed Tomographic Analysis of Bone Microarchitecture in SHP Knockout Mice
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Bae In-Ho
Son Hye-Ju
Kim Jong-Sang
Jeong Byung-Chul
Paek Yun-Woong
Lee Jun-Haeng
Koh Jeong-Tae
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Abstract
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Objectives: Orphan nuclear receptor small heterodimer partner (SHP) is involved in osteoblastic differentiation. This study was undertaken to demonstrate a role of SHP in in vivo bone development using microcomputed tomographic (microCT) analysis of SHP knockout (KO) mice.
Material & Methods: Tibia bones were harvested from 1-, 4-, 8- and 20-week-old wild type (WT) and SHP KO mice. The microarchitecture of tibial bone was analyzed using a microCT (Skyscan 1172; Skyscan, Kontich, Belgium). Samples were scanned at a resolution of 17 μm (isotropic). The X-ray was operated with 50 kV, 200 μA of energy, 1.2 sec of exposure time, and a 0.5 mm thick aluminum filter. Projections were acquired over an angular range of 180°. For quantification of the bone mineral density (BMD), the microCT was calibrated using 2 standard phantoms with densities of 0.25 and 0.75 g/cm3. The image slices were reconstructed and analyzed using CT analyzer software (CTan, Skyscan).
Results: The CT values of tibial trabecular bone were significantly decreased in SHP KO compared to WT at 20-week-old mice determined by microCT; (bone volume / tissue volume [BV/TV, 40%], BMD [80%], and trabecular number [Tb.N, 50%]). However, the CT values were not significantly different between WT and SHP KO in cortical bone. Furthermore, the qualitative indices of trabecular bone such as the structure model index (SMI) and the polar moment inertia (PMI) did not differ between WT and SHP KO mice.
Conclusion: These microCT results supports that SHP may act as a positive regulator of trabecular bone formation.
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KEYWORD
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Bone formation, Microarchitecture, Microcomputed tomography, Small heterodimer partner, Trabecular bone
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